Let’s consider the general trends of development of new sequencing technologies. When a Sanger-based capillary machine that became already a classical sequencing technique returned approximately 70 kb sequences per a run with an average length of DNA reads around 800-1000 bp, The Roche 454 pyro-sequencing technology increased the throughput up to 20 Mb, and the projected throughput of one newly emerging Solexa technology is as much as 10000 Mb per run. However, the length of DNA reads is progressively decreasing to 250 and 25 bp respectively. The expected flood of sequence data, especially from metagenomic studies, therefore poses many new challenges that urgently need attention respecting to both: the sheer amount of data and the rich in complexity.

Now I want present you the approach to deal with the huge amount of sequence data of the length around 800 to 1500 bp that corresponds to the classical Sanger-based sequencing or outputs from Roche 454 after assembling. We have started a new project addressing the analysis of multiple short DNA reads of 20-25 bp. This project exploits completely different approaches. I hope to be able to present in on this meeting next year.