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In the lab we recovered Bacillus
species from opened and unopened jars of garlic mix, beef stew, and vegetable soup. GLC
preformed at our lab identified bacillus subtilis based on the library profiles. The 16 S
ribosomal RNA sequencing confirmed these results. No viable staph aureus were recovered.
The FDA lab reported no staph aureus and a minute quantity of bacillus cereus that would
not cause the toxin mediated illness we saw. The commercial lab assayed for staph
enterotoxin and bacillus cereus diarrheal toxin, which were both negative. They also
reported no heavy metal toxins. There currently exists no practical test for bacillus
cereus emetic toxin, so this could not be tested even though nausea and vomiting were the
predominant symptoms. All patient (ill) and employee nares and stool cultures or rectal
swabs were negative. |